Chytrid fungi construct actin-rich pseudopods, implicating actin regulators WASP and SCAR in an ancient mode of cell motility
نویسندگان
چکیده
The manuscript titled “Chytrid fungi construct actin-rich pseudopods, implicating actin regulators WASP and SCAR in an ancient mode of cell motility” aims to investigate a specific mode of pseudopod-driven cell motility, coined α-motility, and to establish nucleation promoting factors WASP and SCAR/WAVE as its evolutionary signature. While SCAR’s role in pseudopod formation is firmly established, conflicting studies render WASP’s involvement more questionable. A survey of nucleation factors in eukaryotes suggests that pseudopod formation only occurs in organisms with both WASP and SCAR. Therefore, the authors hypothesize that the two factors are required for α-motility. To test this hypothesis, two approaches are used.
منابع مشابه
Chytrid fungi construct actin-rich pseudopods, implicating actin regulators WASP and SCAR in an ancient mode of cell motility
Various cells scattered throughout the eukaryotic tree crawl across surfaces or through threedimensional environments. Evidence now indicates that cell crawling is not a single behavior, but rather a collection of processes, driven by different molecular mechanisms. Understanding these mechanisms and their evolutionary relationships first requires narrowly defining mechanical modes of locomotio...
متن کاملWASP and SCAR are evolutionarily conserved in actin-filled pseudopod-based motility
Diverse eukaryotic cells crawl through complex environments using distinct modes of migration. To understand the underlying mechanisms and their evolutionary relationships, we must define each mode and identify its phenotypic and molecular markers. In this study, we focus on a widely dispersed migration mode characterized by dynamic actin-filled pseudopods that we call "α-motility." Mining geno...
متن کاملSCAR knockouts in Dictyostelium: WASP assumes SCAR’s position and upstream regulators in pseudopods
Under normal conditions, the Arp2/3 complex activator SCAR/WAVE controls actin polymerization in pseudopods, whereas Wiskott-Aldrich syndrome protein (WASP) assembles actin at clathrin-coated pits. We show that, unexpectedly, Dictyostelium discoideum SCAR knockouts could still spread, migrate, and chemotax using pseudopods driven by the Arp2/3 complex. In the absence of SCAR, some WASP relocate...
متن کاملJcb_201704112 1509..1512
1509 The Rockefeller University Press $30.00 J. Cell Biol. Vol. 216 No. 6 1509–1511 https://doi.org/10.1083/jcb.201704112 The growing number of sequenced genomes across a diverse range of organisms has stimulated great interest in phylogenetic analyses of widely expressed proteins with critical roles in cell biology. Such phylogenetic studies of multi-gene protein families have long been used t...
متن کاملWASP family proteins and formins compete in pseudopod- and bleb-based migration
Actin pseudopods induced by SCAR/WAVE drive normal migration and chemotaxis in eukaryotic cells. Cells can also migrate using blebs, in which the edge is driven forward by hydrostatic pressure instead of actin. In Dictyostelium discoideum, loss of SCAR is compensated by WASP moving to the leading edge to generate morphologically normal pseudopods. Here we use an inducible double knockout to sho...
متن کامل